Optimization of Lipase Production by Saccharomonospora azurea AP 11/18 Using Placket-Burman Design and Response Surface Methodology.

Abstract

Optimization of lipase production by Saccharomonospora azurea AP 11/18 was carried out using response surface methodology (RSM). Eight various nutritional parameters were screened using Plackett–Burman experimental design were further optimized by central composite design of response surface methodology for lipase production in submerged fermentation. Among the different carbon sources supplemented, castor oil was most suitable for lipase production while in nitrogen sources, Meat extract was most suitable. The maximum Lipase production of 1041.46 U were achieved in the medium containing Caster oil (1.65g %), Meat extract ( 1.10g%) and Ammonium sulphate (0.06g%) using response surface plots and point prediction tool of Design Expert 8.04 (Stat-Ease Inc.) software.