Simultaneous Estimation of Naturally Occurring Flavonoids by RP- HPLC Method in Dasmoola Churna

Abstract

Flavonoids are pivotal in plant biochemistry and physiology, contributing significantly to the biological activities of plants. Flavonoids are polyphenolic compounds recognized for their antioxidant properties and numerous health benefits. This study aimed to evaluate and quantify the flavonoids catechin, mangiferin, scopoletin, rutin, ellagic acid, baicalein, quercetin, naringenin, kaempferol, chrysin in dasmoola root. High-Performance Liquid Chromatography (HPLC) was used for the simultaneous estimation of flavonoids utilizing a reverse-phase column Optimix C8/C18 with a mobile phase A consisting of Buffer Solution (10 ml of Orthophosphoric into 1000 ml water): Methanol: Acetonitrile in 80: 10: 10 and mobile phase B consisting of 50 :20: 30 ratios at a flow rate of 1 mL/min, with detection at 323 nm. The standard retention times for catechin, mangiferin, scopoletin, rutin, ellagic acid, baicalein, quercetin, naringenin, kaempferol, chrysin were 7.09, 9.52, 21.0, 21.89, 22.64, 25.03, 28.24, 31.43, 34.64, and 37.14 respectively. In the root extract catechin, mangiferin, scopoletin, rutin, ellagic acid, baicalein, quercetin, naringenin, kaempferol, chrysin were detected. These findings confirm the presence of significant flavonoids with potential health benefits in Dasmoola Churna.