Isolation and screening of lignin peroxidase producing microorganism from different geographical location of Andaman and Nicobar island

Abstract

Lignin play an important role in total carbon balance in our earth ecosystem, moreover it is second largest carbon source present in earth environment. There are several ligninolytic enzyme synthesis by microorganism used for degradation of lignin and revert it back in the environment. In the present study lignin peroxidase producing bacterial and fungi were isolated on mineral salts medium supplemented with Lignin.Qualitative determination of lignin peroxidase was done by plate screening method using Guaiacol, Pyrogallol, methylene blue, ramezol brilliant blue and kraft lignin as indicators. Positive cultures were further screened quantitatively in liquid medium using Veratryl alcohol as a substrate. Fungal culture (FSV 3) gave maximum lignin peroxidase production of 131.18 U/ml after 72 hours of incubation.