Collagenase type II inhibitory activity of stem, root and leaf of Cocculus hirsutus

Abstract

Herbal products prepared either from single or multiple botanical ingredients are usually complex and variable in nature. Undoubtedly, the plant kingdom still holds many species of plants containing substances of medicinal value that have yet to be discovered. For this reason, Cocculus hirsutus (L.) a medicinal plant belonging to family Menispermaceae, the perennial climber was selected for the present study. The study was formulated with the objective to assess the Collagenase inhibitory activity and standardization of Cocculus hirsutus. Pharmacognostical study, physicochemical study and Phytochemical study were performed of leaf, stem and the root of Cocculus hirsutus. The leaf, stem and the root of Cocculus hirsutus were extracted with solvents namely ethanol and 70% hydro-alcohol for the determination of Collagenase inhibitory activity. Cocculus hirsutus contained steroids, saponin, flavonoid and phenolics. Stem ethanolic extracts showed highest collagenase inhibitory activity than all other extracts. Again fractionation of stem ethanolic extracts of Cocculus hirsutus was done with petroleum ether, ethyl acetate, n-Butanol and residue. From that petroleum ether and ethyl acetate showed significant collagenase inhibitory activity. So it may conclude that collagenase inhibitory activity may be due to steroids and flavonoids.