Isolation, Characterization and Optimization of Bacteria producing Amylase

Abstract

The present work comprises the amylase enzyme production by isolated amylase producing microorganism. To isolate amylase producing strain, soil samples were collected from different sources. All isolates were screened for amylolytic activity by starch agar plate method. A total of 20 strains of bacteria were isolated which showed amylolytic activity. Out of these, isolate 2a was selected on the basis of maximum hydrolysis for furtherstudy.2a culture was identified as Pseudomonas mendocinaon the basis of morphology and biochemical tests. Effects of temperature, pH, different carbon and nitrogen sources, metal salts and different substrate concentrations of the medium using SmF were optimized. The maximum enzyme production was found after 72 h (1.688 U/min) of incubation at temperature 40°C and pH 7. Among various C and N sources,1% glucose and 1 % Tryptone was found to be best for amylase production. It was found that 0.1% of Mg2+ increased enzyme production whereas other metal ions exhibited inhibitory effects. The enzyme production was maximum at 5% substrate (starch) concentration which shows inducing effect of substrate. These characteristics of Pseudomonas mendocina suggest that this is a promising isolate which merits further investigations for potential applications in various biotechnological processes.